TOOLS SDS-PAGE Loading Buffer (5×)
On the basis of bromophenol blue dye, SDS-PAGE Protein Loading Buffer is a kind of 5X concentrated solution, it contains SDS, DTT, Glycerol, bromophenol blue dye, Tris-HCL Loading buffer. It can be used in SDS－PAGE protein loading of the common protein.
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Wei SC, Tsai CH, Hsu WT, Chao YC. Baculovirus IE2 Interacts with Viral DNA through Daxx To Generate an Organized Nuclear Body Structure for Gene Activation in Vero Cells. J Virol. 2019 Apr 3;93(8):e00149-19.
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Lin WC, Hsu FS, Kuo KL, Liu SH, Shun CT, Shi CS, Chang HC, Tsai YC, Lin MC, Wu JT, Kuo Y, Chow PM, Liao SM, Yang SP, Hong JY, Huang KH. Trichostatin A, a histone deacetylase inhibitor, induces synergistic cytotoxicity with chemotherapy via suppression of Raf/MEK/ERK pathway in urothelial carcinoma. J Mol Med (Berl). 2018 Dec;96(12):1307-1318.
Q1. Why does Tools SDS loading buffer have DTT?
A: 2ME or DTT is usually added into loading buffer as a reducing agent. It can break disulfide bunds and stalbizes protein.
Q2. Can Tools SDSD loading buffer used in native SDS-PAGE?
A: No. It is not recommended.
Q3. Does loading buffer effects downstream experiments?
A: No.
A: 2ME or DTT is usually added into loading buffer as a reducing agent. It can break disulfide bunds and stalbizes protein.
Q2. Can Tools SDSD loading buffer used in native SDS-PAGE?
A: No. It is not recommended.
Q3. Does loading buffer effects downstream experiments?
A: No.